Identificação de aminoácidos por cromatografia gasosa e estudo de um ícone

A seguinte tese de mestrado foi agora livremente disponibilizada online:

Maria Vaz Pinto d’ Avillez, Part I : Comparing derivatization methods for amino acids – one and two-step procedures using gas chromatography – flame ion detector (GC-FID) Part II: Story of an icon from its execution until its conservation, Lisboa, Faculdade de Ciências e Tecnologia da Universidade Nova de Lisboa, 2008.

Está aqui.

Resumo:

Two derivatization methods were tested in twenty-one standard amino acids (AAs) for further identification and quantification by the analytical technique of Gas Chromatography with Flame Ion Detector (GC-FID). For the one-step derivatization reaction N-methyl-N (tert.- butyldimethylsilyl)trifluoroacetamide (MTBSTFA) was used and for the two-step reaction we used hydrochloridic acid (HCl) and trifluoroacetic anhydride (TFAA). We obtained better results with the derivatization procedure in two-steps, although it takes longer, and we concluded that the reproducibility of these reactions is difficult to achieve.